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English: Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides and measurement of fluorescent intensity across the array indicates the abundance of a predetermined set of sequences. These sequences are typically specifically chosen to report on genes of interest within the organism’s genome.[1]
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  1. Lowe, Rohan (2017-05-18). "Transcriptomics technologies". PLOS Computational Biology 13 (5): e1005457. DOI:10.1371/journal.pcbi.1005457. PMID 28545146. PMC: PMC5436640. ISSN 1553-7358.

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5 Oktoobar 2016

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kan hadda12:22, 13 Febraayo 2017Sawirka yar nuuciisa ahaa 12:22, 13 Febraayo 20171,020 × 858 (67 KB)Evolution and evolvabilitytext as paths to avoid artefacts
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08:40, 10 Febraayo 2017Sawirka yar nuuciisa ahaa 08:40, 10 Febraayo 20171,020 × 803 (87 KB)Evolution and evolvabilityreverse order of fragmentation and reverse-transcription.
05:08, 5 Oktoobar 2016Sawirka yar nuuciisa ahaa 05:08, 5 Oktoobar 20161,020 × 858 (66 KB)Evolution and evolvabilityUser created page with UploadWizard

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